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KMID : 0358419650080020025
Korean Journal of Obstetrics and Gynecology
1965 Volume.8 No. 2 p.25 ~ p.30
A Simple and Rapid Diagnostic Method for Vaginal Candidiasis


Abstract
"Candida vaginitis has appeared with increased frequency with the advent of the broad-spectrum antibiotics. The diagnosis is usually not too difficult to make clinically but, occasionally the characteristic lesions are not seen. The physician must then rely on smears and / or cultures or else prescribe `shotgun¢¥ madications with limited efficacy.
Cultures are expensive and during the incubation period the disease continues untreated. Most severe cases can be diagnosed from the wet smear with use of saline but this involves in most cases a tedious and oftentimes fruitless search through the vaginal debris in order to spot the characteristic pseudomycelia. The use of potassium hydroxide instead of saline will dissolve much of this debris but a sharp search for pseudomycelia will still take up valuable time. Cohen, a dermatologist, suggested the addition of Parker ink to potassium hydroxide to facilitate the rapid. diagnosis of fungus infections of skin and nails by staining the mycelia. It was felt that the gynecologist might benefit from this technique in the easier diagnosis of- vaginal candidiasis.
The solution is made by¢¥ adding 10 cc of Parker 51 Superchrome blue-black ink to 20 cc of 20 potassium - hydroxide. One or two drops of the special stains are added to the original smear which is allowed to stand for 5 minutes and then is examined under low and high power. The outlines of pseudomycelia are quite easily seen against a pale blue background.
The gynecologist should be exposed to this rapid and accurate method for office diagnosis of a most, common condition.
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